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  • adding platform information to a bam file

    In using "CountCovariates" for base quality score recalibration, I got a complaint that the platform information was missing. I can force it to default to "illumina" with the "-dP" option, but that doesn't seem wise, and I imagine that more problems will crop up in the future if I do that. I looked in the bwa manual to see if there was an option in sampe (like I've seen for adding read group information), but I didn't see anything. Does anyone know how to add this information?

    Thank you.

    Eric

  • #2
    Hi Eric,

    you have to run AddOrReplaceReadgroups from picard tools for your bams before running the recalibration.

    cheers,
    Sophia

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    • #3
      Originally posted by sdvie View Post
      Hi Eric,

      you have to run AddOrReplaceReadgroups from picard tools for your bams before running the recalibration.

      cheers,
      Sophia
      Thanks very much, Sophia. That worked.

      Eric

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