SEQanswers

Go Back   SEQanswers > Applications Forums > RNA Sequencing



Similar Threads
Thread Thread Starter Forum Replies Last Post
Low DNA input for exome sequencing ymc Sample Prep / Library Generation 8 07-08-2014 03:13 PM
Looking for Illumina Hi Seq sequencing service with low input DNA ajayrav Illumina/Solexa 6 05-13-2014 10:15 AM
Low input directional viral RNA sequencing CottonJack Illumina/Solexa 3 02-11-2013 01:26 PM

Reply
 
Thread Tools
Old 02-17-2017, 01:22 PM   #1
cmuralid
Junior Member
 
Location: Indianapolis

Join Date: Feb 2017
Posts: 1
Default Low input RNA- Stranded sequencing

Hello all,

I am interested in knowing about stranded RNA library prep kits that I could use for low input RNA (as low as 3ng/uL) samples. I was looking at the following kits,

1. SMARTer stranded RNA-seq kit (minimum 100pg input RNA)
2. TruSeq stranded kit (minimum 100ng)
3. NEBNext Ultra RNA kit (minimum 10ng)
4. ScriptSeq V2 kit (minimum 0.5ng)
5. Nugene Ovation kit (minimum 0.5ng)
6. Kappabiosystems Hyperprep kit (unsure about the minimum required)

Other than TruSeq kit, I don't have any idea if any of the other kits are good. It would be great if someone that has used these kits could let me know if they were able to generate good quality data at low input amount.

It would be wonderful if any one could guide me through this.

Thank you very much!
cmuralid is offline   Reply With Quote
Old 02-20-2017, 07:06 AM   #2
MU Core
Member
 
Location: Columbia, Missouri

Join Date: Apr 2008
Posts: 49
Default

You may find this publication helpful.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4310221/
MU Core is offline   Reply With Quote
Reply

Tags
library prep, low input, rna sequencing, stranded rna-seq

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 11:49 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2017, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO