Go Back   SEQanswers > Applications Forums > RNA Sequencing

Similar Threads
Thread Thread Starter Forum Replies Last Post
10X Genomics - no tech support? luc General 4 03-01-2016 12:44 PM
genome sequencing 10x Mr.Zurich1992 De novo discovery 0 02-27-2016 04:31 AM
Is 10x coverage suitable for resequecing 1Gb genome using 454? fusu 454 Pyrosequencing 7 03-07-2009 10:02 AM

Thread Tools
Old 04-09-2017, 02:18 PM   #1
Junior Member
Location: UK

Join Date: Feb 2017
Posts: 7
Default 10x genomics ans spike-in RNAs


I would like to know whether 10x genomics platforsm add RNA spike-in sequences while doing the single cell RNA-seq? I was not able to find out this information online.

I saw few genes with Ercc in the gene list, however I am not getting whether they are spike-in RNAs or not. I have asked 10x genomics tech support but there is no reply, How to know whether spike-in RNAs are used or not.

Your kind help is appreciated.

Rashmi007 is offline   Reply With Quote
Old 04-09-2017, 06:37 PM   #2
Senior Member
Location: Melbourne

Join Date: Jan 2013
Posts: 1,121

10x Genomics does not use spike-in in their current single-cell RNA-Seq workflow.

I wonder what gene list you are reffering to.
nucacidhunter is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 06:21 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2018, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO