SEQanswers

Go Back   SEQanswers > General



Similar Threads
Thread Thread Starter Forum Replies Last Post
28s RNA peak is missing in Human RNA wangwde RNA Sequencing 2 03-20-2017 04:31 AM
MICROBEnrich 28S removal adam.geber Sample Prep / Library Generation 0 02-10-2016 02:36 PM
porcine 28S rRNA sequence? Marianna Goldrick Introductions 2 03-26-2015 12:17 PM
No 28S peak meggie.gr Sample Prep / Library Generation 4 03-07-2014 11:55 PM
Bioanalyzers: High 28S rRNA Peak libprep Sample Prep / Library Generation 6 12-20-2012 03:30 AM

Reply
 
Thread Tools
Old 01-16-2019, 09:47 AM   #1
jbrandim
Junior Member
 
Location: Philadelphia

Join Date: Sep 2011
Posts: 5
Default 28s Missing After gEliminator

I have been extracting RNA from heart tissue for almost 10 years. I usually use the Qiagen RNeasy kit with on column DNAse digestion. This month I tried the RNeasy Plus kit that comes with gEliminator tubes (to remove genomic DNA contamination). When I ran a bioanalyzer to confirm quality I noticed that all the samples I extracted with the gEliminator tubes are missing the 28s peak. Has anyone else ever noticed this?

With gEliminator step
20190116_gElimin.PNG

With RNAse free DNAse on column digestion
Capture.JPG
jbrandim is offline   Reply With Quote
Old 01-17-2019, 06:23 AM   #2
jbrandim
Junior Member
 
Location: Philadelphia

Join Date: Sep 2011
Posts: 5
Default

Update: Talked with Qiagen. The problem seems to be that Qiazol is not compatible with the gEliminator column, because of the acidity.
jbrandim is offline   Reply With Quote
Reply

Tags
28s, bioanalyzer, qiagen, rna, rneasy

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 12:19 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO