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I am trying to prepare samples for down stream ChiP seq. The problem is that I am using mouse tissue and have trouble preparing the samples as there are few protocols for fresh tissue. My question's are
1. Do we fix samples before i homogenize the tissue and how
2. I have used covaris for chromatin shearing but when i am not sure if the SDS in the buffer will affect my downstream sequencing. Without SDS in my shearing buffer (which is same as the covaris kit) i do not see shearing.
3. What kit can i use for preraring the library if my starting material is too low and the samples further go through sorting to get special neuronal cells.
Thanks in advance for the help.
1. Do we fix samples before i homogenize the tissue and how
2. I have used covaris for chromatin shearing but when i am not sure if the SDS in the buffer will affect my downstream sequencing. Without SDS in my shearing buffer (which is same as the covaris kit) i do not see shearing.
3. What kit can i use for preraring the library if my starting material is too low and the samples further go through sorting to get special neuronal cells.
Thanks in advance for the help.