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Old 02-09-2016, 06:44 AM   #1
sanju0891
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Location: germany

Join Date: Mar 2012
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Unhappy Nextera Rapid capture- Reagent reduction

Hello,

I have gone through some posts and found interesting topic regarding the use of half reagents.

We in our lab are doing Nextera Rapid capture enrichment method (custom probes) on the miseq using V2 chemistry. Now we want to reduce our cost since the number of samples are being increased.

so is there any way to reduce the amount of the reagents mentioned in the protocol or change? what steps or concentration of reagents can be modified in the nextera protocol.

If the reagents are reduced to half , will there be any impact on cluster density and sequencing quality itself? how many samples can be multiplexed with reduced reagents?

Any help or suggestions would be appreciated?

Thankyou in advance
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Old 12-29-2016, 09:11 AM   #2
mjg
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Location: Argentina

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Hello sanju0891.
We are also intrested in this topic. I think that it can be done. However we tried this approach only in the tagmentase step.
I don't think that you'll suffer an impact on cluster density if you perform a reliable qPCR step.
I would like to take a look into the post that you are refering to.

Regards
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