this is very useful miRNA discussion. I have some experience using Illumina's flicker tool.. but not much beyond that. mirTools did not work as well as expected and has its shortcomings..
my hypothesis is
fastq -> adapter trimming -> alignment (novoalign?) (to human genome or reference of mirBase?) -> expression
feel free to add to this..
my hypothesis is
fastq -> adapter trimming -> alignment (novoalign?) (to human genome or reference of mirBase?) -> expression
feel free to add to this..
Comment