Hi I am new to NGS. and I have read a lot from your posts. thanks first.
we plan to do NGS from dsDNA for target exome sequencing. the start sample could be 1ng to 1ug, in about 10-20ul.
I now need your advice: which sample prep kit I should pick as so many are out there.
we have Covaris for fragmentation. so mechanical way is not a problem. I do heard nextera can simplify workflow but there is concerns for bias. I personally think nextera is good for low input samples. so I will use standard lib prep workflow first.
I get lost which kit to use. TruSeq DNA Prep kit was said good, but we are hesitated because there are 3 purification steps before lib amplification. And NEB and Kapa all have 1 purification workflow before PCR.
However, what I will miss if i simply choose NEBNext Ultra kit or Kapa Hyper Plus? And is there other kit as better choices? Less cost and good quality are the two main concerns for us.
appreciate your response.
we plan to do NGS from dsDNA for target exome sequencing. the start sample could be 1ng to 1ug, in about 10-20ul.
I now need your advice: which sample prep kit I should pick as so many are out there.
we have Covaris for fragmentation. so mechanical way is not a problem. I do heard nextera can simplify workflow but there is concerns for bias. I personally think nextera is good for low input samples. so I will use standard lib prep workflow first.
I get lost which kit to use. TruSeq DNA Prep kit was said good, but we are hesitated because there are 3 purification steps before lib amplification. And NEB and Kapa all have 1 purification workflow before PCR.
However, what I will miss if i simply choose NEBNext Ultra kit or Kapa Hyper Plus? And is there other kit as better choices? Less cost and good quality are the two main concerns for us.
appreciate your response.
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