Hey everyone. I looked through the forums and this topic hadn't been mentioned in a while, so I hope it's alright to bring it back up.
I'm going to run a ChIP-seq experiment and this will be the first time I'm trying to make my own libraries (Illumina TruSeq ChIP-Seq Kit).
My chromatin was sheared via enzymatic digestion using the Atlantis dsDNAse from Zymo. The chromtain was sheared down to mononucleosomal level (see attached gel picture (1kb+ Ladder, Sample 1, Sample 2, Sample 3).
My question is this: If my DNA is already sheared down to mononucleosomal levels, I can skip the size selection step for the library prep, right? I'm capable of running gels and excising bands, but I have 48 libraries to prep so I'd really rather not do more work if it's not helpful. Thoughts?
I'm going to run a ChIP-seq experiment and this will be the first time I'm trying to make my own libraries (Illumina TruSeq ChIP-Seq Kit).
My chromatin was sheared via enzymatic digestion using the Atlantis dsDNAse from Zymo. The chromtain was sheared down to mononucleosomal level (see attached gel picture (1kb+ Ladder, Sample 1, Sample 2, Sample 3).
My question is this: If my DNA is already sheared down to mononucleosomal levels, I can skip the size selection step for the library prep, right? I'm capable of running gels and excising bands, but I have 48 libraries to prep so I'd really rather not do more work if it's not helpful. Thoughts?
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