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Old 04-23-2015, 07:19 AM   #1
Michal2213
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Location: UK

Join Date: Apr 2015
Posts: 4
Default Ion Proton for allele-specific ChIP-seq, RNA-seq

Hi,

In our lab we are working with allele-specific ChIP-seqs and RNA-seqs. After standard ChIP-seq or RNA-seq protocol we attribute obtained reads to either parental or maternal genome based on available SNP information (there is on avarage 1 SNP per 300 bp).

Our department hosts Ion Proton device - and many researchers use it successfully for regular ChIP-seq and RNA-seq experiments.
I would like to get to know if Ion proton is good enough for my purpose.
Does anybody have experience with allele-specific sequencing on Ion Proton?
Is HiSeq2000 better enough to justify paying 2-3 time more for my experiments?

Best,

Michal
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alle-specific chip-seq, allele-specific rna-seq, ion proton

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