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  • Which PolyA kit to choose?

    Hi,
    For mRNA-Seq, I am looking for a poly(A) selection kit based on oligo(dT) magnetic beads.
    Any recommendations?
    Cheers,
    /Jakob

  • #2
    I have used this with good results:

    The Dynabeads mRNA DIRECT Kit is designed for simple and rapid isolation of pure, intact polyadenylated (polyA) mRNA directly from the crude lysate of animal and plant cells and tissues.

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    • #3
      The NEXTflex Poly(A) Beads from Bioo Scientific work quite well and are very cost-effective. As a disclaimer, I work for Bioo Scientific.

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      • #4
        Thanks for your input Ola and kerplunk412.
        I have ordered the Dynabeads - but will consider NEXTflex beads if the Dynabeads fails:-)
        Cheers, /Jakob

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        • #5
          We used KAPA one. It seems to be cost effective.

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          • #6
            Originally posted by kerplunk412 View Post
            The NEXTflex Poly(A) Beads from Bioo Scientific work quite well and are very cost-effective. As a disclaimer, I work for Bioo Scientific.
            How much? Can you provide a price?

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