It seems like Artemis (release 12.0) translates DNA right ro protein, skipping mRNA stage, so that what seems 2nd reading frame of one chain is actually 2nd frame of complementary one. What is it: i'm wrong, or is this a bug, or is this a feature (and what is its function, if so)?
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Annotations created in Artemis represent the coding sequences of genes. So when the gene is annotated on the forward strand this means that the coding sequence is on the forward strand and that transcription of the gene occurs by reading the gene’s template sequence. So Artemis correctly shows the translation for the coding sequences.
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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04-22-2024, 07:01 AM -
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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