We have prepared a TruSeq DNA PCR-free library, and quantification with Qubit gives roughly 10 times higher concentrations than qPCR (range 4-50 times difference). Could this be because of poor adapter ligation efficiency, and is this normal for a PCR-free library? I was told by the local Illumina rep that such a big difference is not normal. We are using the KAPA Universal qPCR kit.
Jon
Jon
Comment