Epignome Methyl-Seq

[Faria]

Hello Everyone,

Anyone has experience with Epicentre's Epignome Methyl-Seq kit? I recently started using it and my yields have been too low for sequencing. If you have used the kit... I need help optimizing the protocol before I run out of the kit What is your input concentration, number of PCR cycles and yields? Any suggestions would be greatly appreciated.

Thanks in advance

[nucacidhunter]

I have used the kit with 50 ng input following exact kit protocol and libraries yielded around 2 ng/ul. Sequencing result also was good with around 75% mapping to reference.