Dear,
I have performed two runs (targeted sequencing) on miseq using 2x150bp protocol. Unusually, I have obtained fastq containing reads of different length, that during alignment step cause misalignment errors (and a lot of false positives). Any idea for the reason? Any idea to fix it?
I have performed two runs (targeted sequencing) on miseq using 2x150bp protocol. Unusually, I have obtained fastq containing reads of different length, that during alignment step cause misalignment errors (and a lot of false positives). Any idea for the reason? Any idea to fix it?
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