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  • cufflinks accepting BAM files as input???

    After the recent changes in TopHat's output format (BAM instead of SAM), I remember reading here that cufflinks would naturally accept BAM as input.

    However, when I tried it today I did not succeed.

    Do I have to convert the BAM file into SAM? That does not make much sense, given that TopHAt and Cufflinks are part of the same suite ... am I missing something here?

  • #2
    Starting release 0.9.0, Cufflinks and Cuffdiff support both BAM and SAM files. Both file types require that the chromosome names in header @SQ records, if present, must be sorted in the same order as the reads.

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