I have a problem to isolate mRNA with Dynabeads.
I extracted total RNA from Chlamydomonas with AMBION RiboPure kit.
After that, I purified the total RNAs with Qiagen RNeasy mini kit.
And I used Dynabeads to isolate mRNAs but it showed strange picture.
Its picture is attached.
When I tried to make libraries with these samples, I failed. (No bands)
I checked the total RNAs quality by Bioanalyzer 2100 and they are good.
Does anybody have some idea about this problem?
Thanks in advance,
JJ
I extracted total RNA from Chlamydomonas with AMBION RiboPure kit.
After that, I purified the total RNAs with Qiagen RNeasy mini kit.
And I used Dynabeads to isolate mRNAs but it showed strange picture.
Its picture is attached.
When I tried to make libraries with these samples, I failed. (No bands)
I checked the total RNAs quality by Bioanalyzer 2100 and they are good.
Does anybody have some idea about this problem?
Thanks in advance,
JJ
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