Hello everyone!
I'm quite new in this world, so sorry if the question is very naive.
I'm trying to do exome sequencing from FFPE samples, but the company we are working with has some troubles on obtaining a library of good values for Illumina sequencing.
The problem is that on library amplification very often the quantity of amplified library of the right size is quite low. I'm working with quite FFPE old samples (more than 30 years) and I am worried that they can be too much degraded to be sequenced; I have tried to increase the quantity of the starting materials and to increase the purity. I have to increase more this quantity/quality?
Can I give some suggestions to the company?
The kit used to extract DNA is QIAamp DNA FFPE Tissue Kit and the kit to produce the library is Agilen SureSelect V6-Post (as the company said).
Many thanks for all the help!!
I'm quite new in this world, so sorry if the question is very naive.
I'm trying to do exome sequencing from FFPE samples, but the company we are working with has some troubles on obtaining a library of good values for Illumina sequencing.
The problem is that on library amplification very often the quantity of amplified library of the right size is quite low. I'm working with quite FFPE old samples (more than 30 years) and I am worried that they can be too much degraded to be sequenced; I have tried to increase the quantity of the starting materials and to increase the purity. I have to increase more this quantity/quality?
Can I give some suggestions to the company?
The kit used to extract DNA is QIAamp DNA FFPE Tissue Kit and the kit to produce the library is Agilen SureSelect V6-Post (as the company said).
Many thanks for all the help!!
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