Hello,
After hybridisation in the Sureselect protocol, there is a PCR amplification. What program do you use for this step?
The program currently in use is:
98oC at 2 mins
11 cycles of 98oC at 30s/57oC at 30s/72oC at 1 min
72oC at 10 mins
hold at 4oC
The capture size is over 1.5Mb.
Thanks for your help,
Anna.
After hybridisation in the Sureselect protocol, there is a PCR amplification. What program do you use for this step?
The program currently in use is:
98oC at 2 mins
11 cycles of 98oC at 30s/57oC at 30s/72oC at 1 min
72oC at 10 mins
hold at 4oC
The capture size is over 1.5Mb.
Thanks for your help,
Anna.