We are getting only 0.3-0.5 the coverage possible from our MiSeq when sequencing yeast genomic DNA using the Nextera kit for library generation. It seems to be due to tagmentation giving a larger than desired size distribution of fragments. Is yeast DNA a poorer Tn5 target than other DNA? We are using 50 ng DNA (picogreen assay) in the tagmentation reaction, as specified by the protocol. Would reducing the amount of DNA help?
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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04-04-2024, 04:25 PM -
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