Hi,
I have noticed this with all of the recent RNA seq runs we have had with the solexa. The first base error rate is very high (looking at error graphs). Attached is the image for one of the tiles..
do people face the same issue? Any remedies, apart from skipping the first read for eland alignments I suppose!
I think we see this with other solexa runs as well, not just rna-seq..
thanks
I have noticed this with all of the recent RNA seq runs we have had with the solexa. The first base error rate is very high (looking at error graphs). Attached is the image for one of the tiles..
do people face the same issue? Any remedies, apart from skipping the first read for eland alignments I suppose!
I think we see this with other solexa runs as well, not just rna-seq..
thanks
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