Go Back   SEQanswers > Sequencing Technologies/Companies > 454 Pyrosequencing

Similar Threads
Thread Thread Starter Forum Replies Last Post
Doing multiplex in one lane only mcrepeau Illumina/Solexa 27 11-18-2011 09:11 AM
REM e System for enrichment flxlex 454 Pyrosequencing 28 04-27-2011 03:19 AM
Ion Torrent Emulsions / REM e System? niceday Ion Torrent 2 04-12-2011 10:16 AM
16 lane run leakage TonyBrooks 454 Pyrosequencing 1 05-24-2010 08:05 AM
When can I get away without a control lane? lparsons Illumina/Solexa 4 11-02-2009 12:37 AM

Thread Tools
Old 01-04-2011, 06:16 AM   #1
Location: Oslo, Norway

Join Date: Nov 2008
Posts: 415
Default REM e for more than two-lane plates


I previously started a thread on the REM e module for emPCR Enrichment here:, but decided to start a new for this question.

We now have the REM e running on the Hamilton, and it seems to work. However, we had to go through a lot of rounds with 454/Hamilton to get the protocols for 4-lane, 8-lane and 16-lane plates installed. We have been told nobody uses the REM e for 16-lane plates. Is this true? Are we the only crazy ones who are still doing this? Or rather, were doing this (we just decided to run 8 lanes max).
flxlex is offline   Reply With Quote
Old 01-04-2011, 10:00 AM   #2
Senior Member
Location: USA, Midwest

Join Date: May 2008
Posts: 1,177


We don't have a REM e but we completely stopped doing 16 region gaskets simply for practical reasons. They leak terribly and the loss of usable picotiter plate wells is simply too great. We will occasionally do 8 region runs for a submitter doing amplicons (he submits 8 multiplexed pools at a time) but otherwise stick to 4 or 2 region runs, using multiplexing to combine more samples. Multiplexing is far more efficient than running the multiple region gaskets.
kmcarr is offline   Reply With Quote
Old 01-04-2011, 10:16 PM   #3
Location: Oslo, Norway

Join Date: Nov 2008
Posts: 415

Sure, we mulitiplex. But the 16 lanes were for amplicons, just like your 8 lane runs. We just informed our biggest user of 16 lane runs, and they are a bit pissed because of the necessary redesign we now force upon them in the middle of their data collection. Need to talk to them...

But leaky 16 lane gaskets is not something we experience...

Last edited by flxlex; 01-05-2011 at 12:48 AM. Reason: typo
flxlex is offline   Reply With Quote

automation, reme

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 01:48 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO