I've heard from some people that SPRI is not a great way to concentrate DNA products. I usually use 0.6x SPRI on 40-50 uL of PCR product and elute into 30-40uL of water. I am wondering if I could concentrate the product further by just using lower volume of water (or TE, or any elution buffer), like maybe 10uL. Anyone here has any experience doing that? A lot of protocols seem to suggest putting the elutted product on a column to concentrate, which I think kinda defeats the purpose of using SPRI...
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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