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  • Sample sheet for NextSeq-custom

    hi,

    i have a single index run of 16 samples i want to run on the NS. These are not my samples and i was provided with 2 sets of indexes for these samples as they are not too sure what ones they used in setting up the library prep!!!!!

    Anyhow, what can i do? i now have a choice of 32 indexes for my 16 samples. Can i set up my sample sheet to reflect this? Its a custom set of indexes.

    Thanks!

  • #2
    You could just use sample1_index, sample2_index sample32_index type thing to set up a samplesheet to do the demux. Once you know what indexes are real you can do a second run with sampleNames_indexes to clean things up. Ultimately the submitters would need to know what index goes with what sample since there is no way to figure that out automagically.

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