Hello guys!
I'm really beginner at bioinformatics.
I work with viral metagenomics and my library is going to be constructed with Nextera XT for Illumina sequencing. Double barcodes will be used for each pool.
I'm thinking how can I do the quality check of my reads if I have barcodes to identify them. I will use NGSTollkit to make this, but, as I realize, It is impossible to use the Trimming tool because the barcodes needs to stay entire. Is just the QC tool enough?
The best,
Agatha.
I'm really beginner at bioinformatics.
I work with viral metagenomics and my library is going to be constructed with Nextera XT for Illumina sequencing. Double barcodes will be used for each pool.
I'm thinking how can I do the quality check of my reads if I have barcodes to identify them. I will use NGSTollkit to make this, but, as I realize, It is impossible to use the Trimming tool because the barcodes needs to stay entire. Is just the QC tool enough?
The best,
Agatha.
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