Has anyone used Illumina Truseq adapters and the PPC primer cocktail with the Kapa library preparation kit (Illumina compatible version)? The Kapa kit allows the user to use outside adapter and PCR primer primers, this is the reason why I'm asking, if anyone has tried it and how it worked?
Also I am asking because I want to try Kapa's HiFi polymerase. But since I plan to use Truseq adapters, I am at a loss to how to synthesize Truseq PCR primers to use along with Kapa's polymerase, and since Illlumina doesn't publish their PCR primer sequences anymore, I don't know what other PCR primers to use (besides qPCR primers) other than the PPC primer cocktail. Has anyone explored this?
Also I am asking because I want to try Kapa's HiFi polymerase. But since I plan to use Truseq adapters, I am at a loss to how to synthesize Truseq PCR primers to use along with Kapa's polymerase, and since Illlumina doesn't publish their PCR primer sequences anymore, I don't know what other PCR primers to use (besides qPCR primers) other than the PPC primer cocktail. Has anyone explored this?
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