Hi,
I have a fasta file with 20000 transcript sequences. I want to create a gtf file containing the splicing information by mapping them to the genome. I tried using tophat2, but got an error message probably related to the length of the sequences. Any tips on how to make such file?
Jon
I have a fasta file with 20000 transcript sequences. I want to create a gtf file containing the splicing information by mapping them to the genome. I tried using tophat2, but got an error message probably related to the length of the sequences. Any tips on how to make such file?
Jon