Hello,
I'm starting to analyze RNA seq data, and I was hoping you can please help me clear something up
I am using FASTQ groomer, then TopHat2 to align, and then featureCounts, to get a number of reads per gene.
Is the numbers of reads per gene (output) of featureCounts normalized to the gene length?
If not, how can I do that? I want to end up with number of reads per gene, regardless of isoforms etc.
Thanks
I'm starting to analyze RNA seq data, and I was hoping you can please help me clear something up
I am using FASTQ groomer, then TopHat2 to align, and then featureCounts, to get a number of reads per gene.
Is the numbers of reads per gene (output) of featureCounts normalized to the gene length?
If not, how can I do that? I want to end up with number of reads per gene, regardless of isoforms etc.
Thanks
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