Hi, Everybody:
We just completed mRNA seq for some human cells bulk samples.
The QC for those seq data is good. But when we run the alignment for those samples, super low alignment rate come out ( ~50%). We built those lib samples by the pico-input stranded mRNA prep kit from Clontech.
Is there any body have experience for this wired low alignment rate issue?
Is there something wrong about the quality of original samples? or lib prep process? or seq running process?
Any advice would be appreciated.
We just completed mRNA seq for some human cells bulk samples.
The QC for those seq data is good. But when we run the alignment for those samples, super low alignment rate come out ( ~50%). We built those lib samples by the pico-input stranded mRNA prep kit from Clontech.
Is there any body have experience for this wired low alignment rate issue?
Is there something wrong about the quality of original samples? or lib prep process? or seq running process?
Any advice would be appreciated.
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