It sounds like a number of people have been able to prepare successful libraries for sequencing from as little as a few ng of starting DNA. Does anyone have any experience preparing libraries from such small amounts and/or specific tips to increase the chances of success?
I've read some suggestions such as modifying adapter concentrations, increasing the number of PCR amplification cycles and performing PCR before size selection. It seems like there's a lot more information out there for Illumina than for Ion Torrent, including kits such as the Nextera and NEXTflex kits.
Thanks!
I've read some suggestions such as modifying adapter concentrations, increasing the number of PCR amplification cycles and performing PCR before size selection. It seems like there's a lot more information out there for Illumina than for Ion Torrent, including kits such as the Nextera and NEXTflex kits.
Thanks!
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