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Bioo Scientific recently launched the NEXTflex-HT™ Barcodes, single-index barcodes added during the ligation step of library prep, which offer deep multiplexing for both paired-end and single-read Illumina sequencing. Each barcode contains a unique 12 nt index sequence with a Hamming Distance of five or greater, permitting dual error correction to enable proper differentiation between samples. Additionally, each pair of consecutive barcodes is suitable for low level multiplexing. Currently 384 barcodes are available; however, > 2,000 are being developed.
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM