Hi! Friends
We have constructed directional libraries for RNAseq and we are interested in sequencing a range of PCR products (150-1000 bp) for sequencing. Can anyone tell me how much sample should be submitted for illumina sequencing without affecting cluster density? If concentration is in molar terms, how should I calculate this?
Regards
Deepak
We have constructed directional libraries for RNAseq and we are interested in sequencing a range of PCR products (150-1000 bp) for sequencing. Can anyone tell me how much sample should be submitted for illumina sequencing without affecting cluster density? If concentration is in molar terms, how should I calculate this?
Regards
Deepak