Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • questions about index for nextera

    We want to index by ourselves, since we want to pool several 96 well plate thing together. I looked up in the illumina protocol it was written

    Nextera® Index Kit - PCR primers
    5’ AATGATACGGCGACCACCGAGATCTACAC[i5]TCGTCGGCAGCGTC
    (c) i5 Index read -->
    5’ CAAGCAGAAGACGGCATACGAGAT[i7]GTCTCGTGGGCTCGG
    <-- i7 Index read (b)Nextera® Index Kit - PCR primers

    for the i5 and i7 part are there any length restrictions. I saw some said 8 bps, but in others'paper, I saw from 5 bp to 10 bp. I just wondered what is the rule to fill into this i5 and i7 part.

    In addition, can any sequence be filled into these part (of course different sequences) or there have any rules for it.

    Thanks a lot

  • #2
    There are some rules, but even by following them one need to validate designed indices. Nextera XT V2 index kit uses a set of 16x24 indexed primers which has been validated and provides up to 384 multiplexing.

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Techniques and Challenges in Conservation Genomics
      by seqadmin



      The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

      Avian Conservation
      Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
      03-08-2024, 10:41 AM
    • seqadmin
      The Impact of AI in Genomic Medicine
      by seqadmin



      Artificial intelligence (AI) has evolved from a futuristic vision to a mainstream technology, highlighted by the introduction of tools like OpenAI's ChatGPT and Google's Gemini. In recent years, AI has become increasingly integrated into the field of genomics. This integration has enabled new scientific discoveries while simultaneously raising important ethical questions1. Interviews with two researchers at the center of this intersection provide insightful perspectives into...
      02-26-2024, 02:07 PM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, 03-14-2024, 06:13 AM
    0 responses
    32 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-08-2024, 08:03 AM
    0 responses
    71 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-07-2024, 08:13 AM
    0 responses
    80 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-06-2024, 09:51 AM
    0 responses
    68 views
    0 likes
    Last Post seqadmin  
    Working...
    X