Hi all,
I am trying to assemble a bacterial genome with almost 6mb genome size using HGAP 3 from SMRT portal.
I have 10 SMRT cells giving total 64X coverage.
The data has been provided from outside our lab so i dont know the chemistry or sequencing process they used. I run HGAP 3 assembler with mainly default parameter, changing only the genome size and got 240 saffolds which are ver y high. please help me in reducing the scaffold numbers.
Regards
Manjari
I am trying to assemble a bacterial genome with almost 6mb genome size using HGAP 3 from SMRT portal.
I have 10 SMRT cells giving total 64X coverage.
The data has been provided from outside our lab so i dont know the chemistry or sequencing process they used. I run HGAP 3 assembler with mainly default parameter, changing only the genome size and got 240 saffolds which are ver y high. please help me in reducing the scaffold numbers.
Regards
Manjari
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