Hi everyone,
I am going to explain briefly what I'd like to do. I want to see which genes are differentially expressed between plants sterile and fertile. To do so, I will make a massive sequencing (454) of RNA from anthers from each line (fertile and sterile). My question is: what is the protocol to follow to compare the two libraries (fertile and sterile). So, which software I need to use and the steps to follow.
Thanks very much for your attention
I am going to explain briefly what I'd like to do. I want to see which genes are differentially expressed between plants sterile and fertile. To do so, I will make a massive sequencing (454) of RNA from anthers from each line (fertile and sterile). My question is: what is the protocol to follow to compare the two libraries (fertile and sterile). So, which software I need to use and the steps to follow.
Thanks very much for your attention
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