Given the little amounts of DNA obtained after ChIP, are analysis algorithms equipped to normalize data across input/mock/test ips if different amounts of starting material were used in each case to make the libraries?
So if i have 10 ng of IP test, but 40 ng of input and 100 ng of mock, should I necessarily make all libraries from 10 ng only, or can I take all the DNA I have for each of the experiments?
So if i have 10 ng of IP test, but 40 ng of input and 100 ng of mock, should I necessarily make all libraries from 10 ng only, or can I take all the DNA I have for each of the experiments?
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