Hi,
We are planning to use the MethylQuest kit (Millipore) to generate samples for sequencing using the Illumina platform. Has anyone used this kit and if so is there something to keep in mind?
All the literature (as much as I could gather) on MBD-Seq uses the Methylminer kit and I am wondering if there is a reason for that. The end DNA is double stranded and so I do not have to attach the adapters before-hand and the samples can be processed as for Chip-seq, but other than that does anyone have any suggestions for effective MBD-Seq?
Does the MethylQuest protocol have to be modified in any way to achieve good sequencing depth? Also typically what controls are people doing for these kinds of analysis?
Thank you very much.
We are planning to use the MethylQuest kit (Millipore) to generate samples for sequencing using the Illumina platform. Has anyone used this kit and if so is there something to keep in mind?
All the literature (as much as I could gather) on MBD-Seq uses the Methylminer kit and I am wondering if there is a reason for that. The end DNA is double stranded and so I do not have to attach the adapters before-hand and the samples can be processed as for Chip-seq, but other than that does anyone have any suggestions for effective MBD-Seq?
Does the MethylQuest protocol have to be modified in any way to achieve good sequencing depth? Also typically what controls are people doing for these kinds of analysis?
Thank you very much.
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