Tweet
I am in process of preparing libraries with 10 ng and 100 ng mRNA for RNA-Seq. I ran a BioA chip (DNA HS) following second strand synthesis and bead purification. However my BioA traces are flat? At this level of mRNA input would I expect to see a library peak following ds cDNA? I just wanted a quick QC before proceeding along the LC process. Any ideas are welcome!
-
-
Maybe looking at this thread might give you some insight?
http://seqanswers.com/forums/showthread.php?t=2003
-
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...Yesterday, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
Comment