NuGEN announces Encore Whole Blood RNA-Seq Library Systems

[NuGEN]

The Encore Whole Blood RNA-Seq Library Systems provide an end-to-end solution for strand-specific RNA-Seq library construction using as little as 100 ng of total RNA obtained from adult human whole blood. The integrated workflow from input of total RNA to fully constructed library is highly reproducible, and can be completed in just 7 hours. The core technology used in this product enriches for non-rRNA and non-globin sequences in NGS libraries using NuGEN’s Insert Dependent Adaptor Cleavage (InDA-C) technology for targeted elimination of selected library sequences. The result is a significant reduction in sequencing reads derived from human rRNAs and adult human reticulocyte globin (HBA1, HBA2, HBB, and HBD) genes.

The Encore Whole Blood RNA-Seq Multiplex System 1-8 and 9-16 each provide eight unique barcoded adaptors to enable multiplex sequencing to further optimize efficiencies and cost savings in transcriptome sequencing.

http://www.nugeninc.com/nugen/index.cfm/products/encore/encore-wb/

[NuGEN]

Data Sheet now available

The Data Sheet for the The Encore Whole Blood RNA-Seq Library Systems is now available on the NuGEN web site:

http://www.nugeninc.com/nugen/index.cfm/linkservid/A9826E58-0548-431E-9F5B117A1E36CFC0/showMeta/0/

This document shows results produced with four independent PAXgene samples obtained from two different research groups. Sequencing metrics reported include %rRNA and %globin aligned reads, read coverage, transcript detection, and RefSeq strand retention.