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  • short reads are not adapter sequences, why?

    I found some short reads in my results. They are apparently shorter than normal reads ( for example ~15 bp vs. 51 bp). But, I found they are not adapter sequences. Why? As I understand, if the inner cDNA is short, the reads should end in 3' adapters, right?

  • #2
    Reads from any Illumina runs are all the same length unless they have been trimmed. I suspect that sequencing centre has already trimmed the adapters resulting in some short reads.

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