Hi,
We have one question,
We tried using nextera for fragmentation of bacterial DNA genomic , but this non fragmented completely.
We change the concentrations of DNA initial, time of fragmentation, with kit NEBNext, sonication, and for heat ,but nothing...
The best result was one library with average of 1368 pb (with nextera), we are thinking use this samples with a kit of 500 cycles, but we don´t know if this will result correctly.
Help me please!!!
Thanks!
We have one question,
We tried using nextera for fragmentation of bacterial DNA genomic , but this non fragmented completely.
We change the concentrations of DNA initial, time of fragmentation, with kit NEBNext, sonication, and for heat ,but nothing...
The best result was one library with average of 1368 pb (with nextera), we are thinking use this samples with a kit of 500 cycles, but we don´t know if this will result correctly.
Help me please!!!
Thanks!
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