Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Error in cdna for newMapping

    I followed the following directions for aligning 454 reads to an assembled rna-seq dataset.
    newMapping projdir
    addRun [options: -p, -lib, -mcf] [projdir] filedesc
    removeRun [projdir] filedesc
    setRef [options: -gref, -cref, -random] [projdir] refdesc
    runProject [options] [projdir]

    I got the following error when I tried to use the -cref option with setRef
    my command:
    setRef -cref DH1 /fullpathname/grossSilber.fa
    error:
    Error: File is not a fasta file of sequence: -cref

    Is there a particular format for a cref fasta?

    It worked when I setRef with no options, but of course runProject won't work with -cdna

    Any helpful hints?

  • #2
    Originally posted by prettyPeas View Post
    I got the following error when I tried to use the -cref option with setRef
    my command:
    setRef -cref DH1 /fullpathname/grossSilber.fa
    error:
    Error: File is not a fasta file of sequence: -cref
    I am pretty sure the problem is the 'DH1' between the -cref and the path. -cref expects only a file, or the path to a file.

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Recent Advances in Sequencing Analysis Tools
      by seqadmin


      The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
      Today, 07:48 AM
    • seqadmin
      Essential Discoveries and Tools in Epitranscriptomics
      by seqadmin




      The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
      04-22-2024, 07:01 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, Today, 07:17 AM
    0 responses
    7 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 05-02-2024, 08:06 AM
    0 responses
    19 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-30-2024, 12:17 PM
    0 responses
    20 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-29-2024, 10:49 AM
    0 responses
    28 views
    0 likes
    Last Post seqadmin  
    Working...
    X