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Old 07-03-2018, 12:54 PM   #1
hoytpr
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Location: Stillwater

Join Date: Dec 2009
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Default Opinion needed on Truseq vs Scriptseq

So quite simply, when doing the math, 48 bacterial RNA-seq samples (libraries) would cost over a thousand bucks more using Truseq + Ribozero, versus using ScriptSeq "complete" + Scriptseq "barcodes" + Lucigen's Failsafe PCR mix. (for this small core Illumina discounts don't even cover shipping).

This amount of money is significant to the researcher. But we haven't used the ScriptSeq and already have all the DUI's for the TruSeq kits.

Can I get a few opinions about TruSeq vs ScriptSeq? Could we safely use the ScriptSeq kits? Even if one does everything correctly, will these kits cluster about the same?

Thanks
-pete
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