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Hey folks,
Lab tech who's very new to this business, so please bear with me.
I've been tasked with creating DNA libraries using Agilent's Sure Select Strand Specific RNA library prep kit, and then further processing them using the Sure Select XT Target Enrichment system.
I've hit a rather large snag at the hybridisation and capture step. I have nowhere near the recommended input DNA of 750ng (protocol requires 3.4ul at 221ng/ul), at best I have around half the total input that's required.
Is it possible to run with this? Or should I go back to my library creation step and see if I can increase the DNA yield without introducing too many PCR artefacts?
Cheers
Craig xx
Lab tech who's very new to this business, so please bear with me.
I've been tasked with creating DNA libraries using Agilent's Sure Select Strand Specific RNA library prep kit, and then further processing them using the Sure Select XT Target Enrichment system.
I've hit a rather large snag at the hybridisation and capture step. I have nowhere near the recommended input DNA of 750ng (protocol requires 3.4ul at 221ng/ul), at best I have around half the total input that's required.
Is it possible to run with this? Or should I go back to my library creation step and see if I can increase the DNA yield without introducing too many PCR artefacts?
Cheers
Craig xx