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Old 10-07-2016, 08:42 AM   #1
Vinz
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Default Interested in reviewing our manuscript on amplicon sequencing?

We started HLA genotyping by amplicon sequencing in 2013. 2.7 million samples and several thousand MiSeq/HiSeq runs later, we destilled some quite interesting lessons learned from the data.
Our manuscript is ready to be reviewed at peerage of science . Since some of the experienced amplicon sequencers post here, I thought I ask for a review here.
Would be greatly appreciated if you could give it a fair review!
(2.7 million samples genotyped for HLA by next generation sequencing: Lessons learned)
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Old 10-07-2016, 08:52 AM   #2
thermophile
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I'm interested but your link doesn't take me to your paper and I'm not seeing how to search for it.
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Old 10-07-2016, 10:28 AM   #3
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Ditto. There is no way to search by title of the paper (only by author name or so it appears).
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Old 10-08-2016, 01:41 PM   #4
Vinz
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Sorry, it is our first paper with peerageofscience and I am not yet familiar with the way to refer to manuscripts.
Keywords are: Next generation sequencing; HLA genotyping; high resolution, high throughput; amplicon PCR; primer dimers; PCR chimerism; novel alleles
Authors: Schoefl, Quenzel, Lange
If you can't find it, send me a message with your name (after registering there) and I could suggest you as an reviewer. Thanks!
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Old 10-12-2016, 01:00 AM   #5
Dario1984
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HLA typing is currently a popular genomics topic. You should submit your work to a traditional journal as soon as possible.
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Old 02-16-2017, 04:05 AM   #6
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Our manuscript got published by BMC genomics. The peerageofscience experience was bumpy as currently there seems not to be a genomics community at peerageofscience. So at the end we went the conventional peer review route.
If you are doing targeted short amplicon NGS - enjoy the read!
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Old 02-16-2017, 08:40 AM   #7
thermophile
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fantastic! I've been looking forward to reading this.
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Old 02-16-2017, 06:00 PM   #8
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When will the 1919 novel alleles be added to the IMGT/HLA database?
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Old 02-17-2017, 01:25 AM   #9
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We are working on this. The current release (3.27) contains 606 of those alleles.
However, we are not in favor of submitting partial sequence information for novel alleles. Therefore, we always characterize the full genes from UTR to UTR. This obviously is more challenging, but should be worthwhile. This project is ongoing... A manuscript describing this should be available in the next weeks in the journal HLA.
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