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Thread | Thread Starter | Forum | Replies | Last Post |
Illumina HiSeq library insert size | SDPA_Pet | Bioinformatics | 21 | 03-08-2017 07:51 AM |
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#1 |
Senior Member
Location: 63130 Join Date: Apr 2012
Posts: 122
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Hello,
I got some illumina data which obviously has very small insert size as many of the read pairs have >95% overlap. I went back to illumina library protocol and feel that it generally suggests a library insert size longer than the total length of the paired-end read, that is, read pair does not overlap. What is the optimal library insert size? Should I move forward for data analysis? Thanks a lot! |
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#2 |
Senior Member
Location: East Coast USA Join Date: Feb 2008
Posts: 6,700
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What kind of an experiment is this? It may still be fine to use that data after appropriate trimming. You could optionally merge.
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#3 |
Senior Member
Location: 63130 Join Date: Apr 2012
Posts: 122
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It is metagenomic data from fecal samples. I did trim the samples with trimmomatic.
Last edited by capricy; 03-11-2017 at 07:46 PM. |
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