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Old 02-21-2012, 10:09 AM   #1
Juergen F
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Location: California + Germany

Join Date: Jan 2012
Posts: 3
Default Questions sample prep for virus genotyping sequencing

Hi,

Questions:

0 – How do I guarantee a certain # of redundant reads per virus target gene area to be utilized later in assembly?

1 - I assume I would need a virus extraction method (Qiagen, Roche) performed upfront prior starting e.g. an Illumina multiplexing workflow with library prep?

2 - Which sample prep methods do match the input bp size requirements best (... output bp size of sample preps)?

3 - What input virus concentration (copies/ IU) do I need to start e.g. the Illumina multiplexing workflow?

Thanks & Greetings
Juergen
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