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Old 01-14-2010, 07:19 AM   #1
hitdavid
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Location: China

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Smile help! what is a paired-end read?

I am a newer in this area.
Could anybody tell me what is a paired-end read?

someone told me that a paired-end read is a read with the two 3' end sequences were sequenced.

for example: a fragment of DNA strands like this

3' 5'
ATTTGCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXATTCGG
5' 3'

and this is used in an assembly process when sequencing.

I am confused by his answer.
Is his answer right? if not, please give me a right answer!
Thank you N*very much!
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Old 01-14-2010, 11:42 AM   #2
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