SEQanswers

Go Back   SEQanswers > Introductions



Similar Threads
Thread Thread Starter Forum Replies Last Post
MinION protocols? JBKri Oxford Nanopore 32 06-07-2017 01:40 PM
minION throughput tsangkl Oxford Nanopore 3 10-09-2016 11:52 AM
Cost of MinIon? bios90 Oxford Nanopore 1 12-16-2015 12:52 PM
MinIon reads mido1951 Oxford Nanopore 13 10-28-2015 07:55 AM
PacBio vs MinION BioKiwi Bioinformatics 7 06-23-2015 01:48 AM

Reply
 
Thread Tools
Old 12-13-2016, 11:47 AM   #1
shayrabedyan
Junior Member
 
Location: Bulgaria

Join Date: Dec 2016
Posts: 3
Smile MinION enthusiast

Hi there,

I am new to this forum, and I am a MinION enthusiast. I hope to start testing the technology very soon, and that it will live up to my expectations.

Best!
shayrabedyan is offline   Reply With Quote
Old 12-22-2016, 09:53 AM   #2
gringer
David Eccles (gringer)
 
Location: Wellington, New Zealand

Join Date: May 2011
Posts: 786
Default

One way to get the MinION to live up with your expectations is to look at where the technology was about 6 months ago, and base your expectations on that. Conveniently, this is about the time it takes for a quick MinION experiment to be carried out and publications peer reviewed.
gringer is offline   Reply With Quote
Old 12-22-2016, 09:59 AM   #3
usad
Member
 
Location: aachen

Join Date: Sep 2009
Posts: 53
Default oxford now

Oxford nanopores changed dramatically in output a couple of months ago. Have a look at the data prereleases. 2-7 Gbases per run was normal for us (with a bit of experience)
Error rate is of course still very high.

Best Wishes
Björn
usad is offline   Reply With Quote
Old 12-22-2016, 10:55 AM   #4
gringer
David Eccles (gringer)
 
Location: Wellington, New Zealand

Join Date: May 2011
Posts: 786
Default

They've been changing dramatically in output and read quality for at least the last year. R7.3 was in use at the start of this year, and that had an output of 200-400Mb with good sample prep and training.
gringer is offline   Reply With Quote
Old 12-23-2016, 11:35 AM   #5
shayrabedyan
Junior Member
 
Location: Bulgaria

Join Date: Dec 2016
Posts: 3
Default

Quote:
Originally Posted by gringer View Post
One way to get the MinION to live up with your expectations is to look at where the technology was about 6 months ago, and base your expectations on that. Conveniently, this is about the time it takes for a quick MinION experiment to be carried out and publications peer reviewed.
Thanks for the feedback! I am really enthusiastic as this instrument is really affordable and the progress in base calling analysis and chemistry/pore changes promise real use very soon. I ordered one these days, and I plan to use it for RNA-seq and maybe targeted gDNA and Chip-seq scenarios. I just don't have access yet to the community to check on the protocols and requirements. I guess I will have it after the Holidays.
shayrabedyan is offline   Reply With Quote
Old 12-23-2016, 11:40 AM   #6
shayrabedyan
Junior Member
 
Location: Bulgaria

Join Date: Dec 2016
Posts: 3
Default

Quote:
Originally Posted by usad View Post
Oxford nanopores changed dramatically in output a couple of months ago. Have a look at the data prereleases. 2-7 Gbases per run was normal for us (with a bit of experience)
Error rate is of course still very high.

Best Wishes
Björn
Thanks for the reply! Have you tried the new neural network algorithm? I am curious if 1D protocol could do if more sampling is done on same source material to increase the coverage.
shayrabedyan is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 07:35 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2017, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO