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Old 02-08-2017, 06:23 AM   #1
MisUser
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Question Maximum number of cycles for kit Miseq 50 cycles

Hi sequencing Masters!

What is the maximum number of cycles we can do with a Miseq V2 50 cycles kit. I have been told that there is enough reagents for more than 55 cycles including the 6 cycles for index reading. Can someone confirm this information?
Thank you very much for your support. It is very appreciated.
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Old 02-08-2017, 06:26 AM   #2
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How many are you looking to get?
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Old 02-08-2017, 06:28 AM   #3
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54 maximum + 6 for index
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Old 02-08-2017, 06:32 AM   #4
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It should be fine. Local core does 66 cycle (50x8x8) runs off and on.
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Old 02-08-2017, 06:48 AM   #5
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Great news. Thank you for your answer Genomax!
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Old 02-08-2017, 11:33 AM   #6
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I got an answer from Illumina's tech support:
MiSeq v2 contains enough reagents for 75 cycles of chemistry
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Old 02-14-2017, 09:34 PM   #7
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here you can find the answer:

https://support.illumina.com/bulleti...in-my-kit.html
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Old 10-18-2017, 09:03 PM   #8
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I know this is late but we routinely do 78 cycles with the MiSeq v2 50-cycle kit. (31 read1, 31 read2, 8 index1, 8 index2).
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Old 10-18-2017, 10:28 PM   #9
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Plus 7 dark cycles used for index2 read so 85 cycles in total. This seems to be 10 cycles more than Illumina specification.

Last edited by nucacidhunter; 10-18-2017 at 10:30 PM.
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